Зборник радова

на свим локалитетима указују на интензивне процесе биодеградације. Липолитичке бактерије нађене су у свим узорцима осим у води извора Граца и Марецове реке. То указује на вероватно мали удео липида у органским материјама прнсутним у води на ова два локалитета. Према резултатима извори Граца и Марецове реке не садрже колиформне и фекалне бактерије за разлику од осталих испитиваних извора, који показују известан степен загађења. Укупно је константовано 11 група макроинвертебрата. На испитиваним изворима доминирају Chironomidae, Mollusca, Gammaridae н OHgochaeta. Инсектске групе Ephemeroptera, Trichoptera, Coleoptera, Diptera и Plecoptera су нешто мање заступљене, док су најмалобројније Hinidinea и Planaria (Trichlada). Обзиром да рад обухвата само летњи аспект (јул-август 1993. године) добијени резултати могу послужити као основа за дугорочнија истраживања крашких извора. ABSTRACT Springs are different from the other parts of water ecosystems according to their geomorphological, physical and Chemical characteristics and lo their biocenosis. This work includes a complex of microbiologica! researchcs and investigations of benthos fauna of six karst springs. Three of them arc sltuated in Bor region, zone of mountains of Kučaj, and three are in Valeevo region, zone of river basein of Kolubara. Collected material was analysed in laboratories of Zaječar Public Health Institute Timok" and at the Science Center Petnica. The aim of this work was the detection of bacteriologica! polution and the presence of organic substances in the water samples. That was done on the base of presence of different physiological groups of bacteria. The composition of fauna of benthos of investigated karst springs was also analysed. Sampling procedure and bactcriological analyses were according to Standard microbiological methods. On the basis of the obtaincd results it can be coucluded that the spring parts of Banja, Pocibrava and river of Mikulj contain coliform bacteria and the highest MPN was 3.8x103 cells/1 of a sample taken at the spring of Pocibrava. Streptococcus faecalis was identified in the springs of Banja, river of Vojal and Mikulj. Presence of coliform and fecal bacteria in the spring of Banja was expected because of the farms situated near the spring. Proteus sp. arc identified in the spring of Pocibrava. Sulphite-reducing clostridia were found in the springs of Banja and Pocibrava only. Their presence indicates the anaerobic conditions caused by intensive metabolic processes in the water. Presence of starch-, cellulose- and proteins-decomposing bacteria at all the localities indicates intensive processes of biodegradation. Lipids-decomposing bacteria were found in all the samples exept in water samples of the spring of Gradac and river of Marec. That indicates the small lipids quantities in water of these two localities. Obtaincd results show that the springs of Gradac and river of Marce do not contain coliform and fecal bacteria. The other analysed springs