Chinese Journal of Physiology

42 H, C, HOU anp R. K. S. LIM

response by electrical stimulation of the splanchnic nerve, in which the vasomotor fibres have been caused to degenerate by section 5 days previously. This would indicate that secretory fibres to the stomach travel in both vagus and sympathetic nerves. The existence of inhibitory fibres in the vagus has been demonstrated by Pavlov and his school, and in the sympathetic by Ishido (5), although the inhibitory effect (on meal stimulation) is slight. Except for the inhibitory effect of the vagus, the influence of nerves on the basal secretion is not definitely known.

METHODS

The following types of gastric preparations have been employed. All pouches consist of about one-sixth of the whole stomach,

1. Fistulc-pouch. This is a modified Pavlov-pouch constructed as follows. A longitudinal incision about 5 cm long is made in the ventral wall of the stomach in the position indicated in fig. 1. The gastric cavity opened and the mucosal incision carried round the stomach along a line parallel to the fundic part of the lesser curvature. The cut edges of the mucosa are then sutured so as to form two separate gastric cavities, one in normal continuity with the rest of the alimentary tract and the other completely shut off. This outer chamber is fistularized, In this preparation all nerves are preserved and there is a minimum of injury to the enteric nerve plexus.

2. Reverse-Pavlov-pouch. This differs from the usual Pavlovpouch in that it is made in the reversed way. Instead of making the cut in the stomach from the pyloric region towards the cardiac end, the greater curvature is incised from the cardiac dome towards the pylorus (see fig. 2). In this way it was hoped to have a preparation lacking only in vagal fibres and retaining the enteric and sympathetic connexions. 8. Pavlov-pouch. (See 9, fig. 2).

4. Heidenhain-pouch. This pouch has neither vagal nor enteric connexions, but again some fibres from the dorsal vagus may be present, if the pouch is constructed from the upper fundus region (see 9, fig. 3).

5. Auto-transplanted-pouch. This is a Heidenhain-pouch cut away entirely from every connexion and replanted by vessel anastomosis (see fig. 3). It is of course completely denervated, but contains the Auerbach and Meissner plexuses included within its walls.