Chinese Journal of Physiology

Chinese Journal of Physiology, 1929, Vol, III, No. 1, pp, 29—40,

THE RELATION OF THE MITOCHONDRIA-GOLGI COMPLEX TO SECRETION Ill. PHYSIOLOGICAL IDENTIFICATION OF THE VITALLY STAINED MITOCHONDRIA-GOLGI MATERIAL

WEN-CHAO MA, HSI-CHUN CHANG anp AN-CH’ ANG LIU

(From the Departments of Physiology and Anatomy, Peking Union Medical College, Peping)

Received for publication Sept. 25, 1928

In a previous paper (29) the technique of intravital staining with neutral red and Sudan III was given as a method for demonstrating the Golgi material. The aim of the present communication is to substantiate this claim by showing that the neutral red and Sudan III stained material corresponds to the osmiophile material obtained by the impregnation technique, in the resting and active gland, when simultaneous specimens are taken from the same tissue.

MATERIAL

The pancreas of rabbits and dogs was studied. Under urethane (for rabbit) or veronal (for dog) anaesthesia, the mixture of neutral red and Sudan III was injected and the pancreatic ducts cannulated. Small, thin pieces of pancreas were removed with scissors, partly for fresh examination and partly for osmium impregnation. It was found that removal of tissue in between ligatures for haemostasis would seriously alter the normal position of the Golgi-mitochondria material. Bleeding was fully checked after excision of the tissue sample. A sketch of the fresh preparation was recorded for future reference and comparison, The tissue had to be handled with great care in order to preserve the normal topography of the intracellular structures. Pilocarpine was given to excite activity, and pancreatic juice collected to control the degree of activity evoked. Tissues were removed periodically during the height and at the end of secretion. Similar observations were made on the salivary glands for the purpose of comparison.

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